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HPF™ Fluorescence Filters - http://newportfranklin.com/products/fluorescencefiltersets/ffsets.htm • Patented Stabilife Coatings Newport's HPF series of filters for fluorescence detection is a compilation of product designs of filters to be used with many of the most popular standard fluorophores and some of the most promising new fluorophores. HPF filters are manufactured using Newport’s patented Stabilife coating technology which provides excellent spectral performance, spectral stability, and physical durability. Stabilife HPF filters can be supplied in a variety of configurations including a traditional laminated multi-component format, an air-spaced multi-component format, a single substrate optic quality element (for imaging applications), and a single thin substrate, for dicing into microchip format for micro-array applications. |
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High Signal-to-Noise Fluorescence Filters • High transmission Newport’s High Signal-to-Noise Fluorescence Filters are designed for use in systems where high signal-to-noise ratios of each filter must be established independently. Many fluorescence detection filters, being developed for epi-fluorescence microscopy applications, depend upon pairing to achieve pair-specific signal-to-noise ratios. High S/N fluorescence filters do not have this limitation. With a typical non-paired signal-to-noise ratio of 100,000:1, any High S/N filter can be paired with any other filter in the series, and deliver the same exceptional pair signal-to-noise ratio as would have been achieved with its traditional pair. The filters are designed with bandwidths consistent with the absorbance or emission spectra of the target fluorophore, but narrower to accommodate non-traditional pairing. Using a multi-cavity film design and multiple blocking components, typical spectral rejection (blocking) of <_1.0 x 10-8 is achieved from UV to 925nm. This provides integrated blocking or ->OD5 when used with a tungsten source operating at 2800 °K and an S20 or equivalent photocathode detector. These filters are a key enabling technology in numerous fluorescence based research and clinical instruments. Typical applications include instrumentation for flow cytometery, drug discovery, genetic analysis, and other fluorescence-based instrumentation. |
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